APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment of human PCSK9.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
proprotein convertase subtilisin/kexin type 9 , FH3 , HCHOLA3 , LDLCQ1 , NARC-1 , NARC1 , PC9
Cellular Localization
Cytoplasm,Secreted,Endosome,Lysosome,Cell surface,Endoplasmic reticulum,Golgi apparatus
Background
This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an autocatalytic processing event with its prosegment in the ER and is constitutively secreted as an inactive protease into the extracellular matrix and trans-Golgi network. It is expressed in liver, intestine and kidney tissues and escorts specific receptors for lysosomal degradation. It plays a role in cholesterol and fatty acid metabolism. Mutations in this gene have been associated with autosomal dominant familial hypercholesterolemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]
Database
Research Area
DATA IMAGES
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GTX639955 ICC/IF Image
PCSK9 antibody [HL2814] detects PCSK9 protein at Golgi apparatus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PCSK9 stained by PCSK9 antibody [HL2814] (GTX639955) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
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GTX639955 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PCSK9 antibody [HL2814] (GTX639955) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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REFERENCE
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REVIEW
PCSK9 antibody [HL2814]
Cat. No. GTX639955
Rating
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( Average 5 based on 1 users reviews)
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Application
Western Blot(WB)
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( Average 5 based on 1 users reviews)
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Date :
Anonymous submitted on 20-Mar-2024
Application Tested :
WB
Sample Species :
Hu
Sample :
HeLa cells with/without PCSK9 KO treatment
Amount used :
30μg
Primary Antibody :
1:1000, 4°C, >12Hr